Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor

Title:

Retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells is potentiated by leukemia inhibitory factor

Creator:

Pacherník, J., Bryja, V., Ešner, M., Hampl, A., and Dvořák, P.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:4a00a0aa-b9b7-4c08-ac3d-a06afd117046
uuid:4a00a0aa-b9b7-4c08-ac3d-a06afd117046

Subject:

Neural differentiation, Leukemia inhibitory factor, Retinoic acid, and Embryonal carcinoma cells P19

Type:

article, model:article, and TEXT

Description:

Leukemia inhibitory factor (LIF) is a cytokine that exhibits proliferation, survival and differentiation in a wide range of cell types. Here we show that LIF potentiates retinoic acid-mediated neural induction in pluripotent P19 embryonal carcinoma cells. This activity of LIF was demonstrated by a profounded neural morphology followed by increased expression of neural-specific proteins (N-CAM, III β-tubulin, and GAP-43), up-regulation of early neural lineage-specific gene Mash-1, and down-regulation of early endoderm-specific genes α-fetoprotein and GATA-4. Moreover, LIF also slows growth and increases the level of apoptosis in differentiating cells.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2005 Volume:54 | Number:2

Harvested from:

CDK

Metadata only:

false