Measurement of cellular excitability by whole cell patch clamp technique

Title:

Measurement of cellular excitability by whole cell patch clamp technique

Creator:

Karmažínová, M. and Ľubica Lacinová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:48753c8f-cbfd-4c09-8cb4-5bd0ccb28b9b
uuid:48753c8f-cbfd-4c09-8cb4-5bd0ccb28b9b

Subject:

Fyziologie člověka a srovnávací fyziologie, genetika, molekulární genetika, genetics, molecular genetics, patch clamp, calcium current, activation, deactivation, inactivation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion channels or through the whole assembly of ion channels expressed in the whole cell. Whole cell configuration is more common for measurement of ion currents and the only one enabling measurement of action potentials. This method allows detailed analysis of mechanisms and structural determinants of voltagedependent gating of ion channels as well as regulation of channel activity by intracellular signaling pathways and pharmacological agents., M. Karmažínová, L'. Lacinová., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false