High irradiance effects on the xanthophyll cycle pigments and the activity of violaxanthin de-epoxidase in soybean callus

Title:

High irradiance effects on the xanthophyll cycle pigments and the activity of violaxanthin de-epoxidase in soybean callus

Creator:

Pandey, D. M., Kim, K. H., Kang, K. H., and Yeo, U. D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2f906d28-000e-427b-b610-7ea374d8d799
uuid:2f906d28-000e-427b-b610-7ea374d8d799
issn:0300-3604
doi:10.1023/B:PHOT.0000040584.93955.a1

Subject:

antheraxanthin, Glycine max, HPLC, neoxanthin, violaxanthin, and zeaxanthin

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

High irradiance (HI) effects on xanthophyll cycle pigments (XCP) and activity of violaxanthin de-epoxidase (VDE) in terms of de-epoxidation index (DEI) were studied in soybean calli. The calli from the hypocotyl segments of 5-d seedlings were induced on a solid (1.1 % agar) MS medium (pH 5.8) supplemented with 4.52 μM 2,4-dichloro-phenoxyacetic acid, 2.32 μM kinetin, and 3 % sucrose. After a 30 d cultivation, the green calli were irradiated for 24 h with "white light" (HI, 1 300 μmol m-2 s-1) and VDE was isolated from the photosystem 2 (PS2) particles. In the control (0 h irradiation) callus, the reaction of PS2 particles with VDE in the presence or absence of Tween 20 resulted in the decrease of VIO content and the increase of ZEA content. In the 24 h HI-callus, the reaction of PS2 particles in the absence of VDE led to the decrease of VIO and ANT contents and increase of ZEA content. In the control, DEIs in the presence of VDE with or without 0.1 %Tween 20 (1.04 and 1.06, respectively) were significantly higher than the DEI (0.76) in the absence of VDE. In the HI-callus, DEIs in the presence of VDE with or without 0.1 %Tween 20 (0.98 and 0.96, respectively) were similar to that (1.03) in the absence of VDE. and D. M. Pandey ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

153-156

Source:

Photosynthetica | 2004 Volume:42 | Number:1

Harvested from:

CDK

Metadata only:

false