Long-term peritoneal dialysis treatment provokes activation of genes related to adaptive immunity

Title:

Long-term peritoneal dialysis treatment provokes activation of genes related to adaptive immunity

Creator:

Parikova, A., Hruba, P., Krediet, R. T., Krejcik, Z., Stranecky, V., Striz, I., and Viklicky , O.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2cec55a5-da66-43f6-81f4-a2b5beae1457
uuid:2cec55a5-da66-43f6-81f4-a2b5beae1457
doi:10.33549/physiolres.934158

Subject:

peritoneal dialysis, gene expression, and peritoneal membrane alterations

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Permanent irritation of the peritoneum during peritoneal dialysis (PD) treatment leads to local chronic inflammation and subsequently activation of processes driving fibrogenesis in the long-term. The aim of the study was to compare the peritoneal effluent transcriptome of 20 patients treated less and 13 patients treated more than 2 years using microarray analysis. An increased expression of genes associated with an immune response was observed in long-term treated patients with well preserved peritoneal function, when compared to patients treated less than 2 years. From 100 genes highly expressed in long-term patients, a significant up-regulation of six was found by RT-qPCR: LY9 (lymphocyte antigen 9), TNSFR4 (tumor necrosis factor receptor superfamily, member 4), CD 79A (CD79a molecule), CCR7 (chemokine C-C receptor 7), CEACAM1 (carcinoembryonic antigen-related cell adhesion molecule 1) and IL2RA (interleukin 2 receptor alpha chain). Furthermore, the effluent cell population was analysed. A positive relationship between the number of granulocytes and NK cells on one hand, and duration of PD treatment on the other, was shown. We conclude, that the mechanisms of adaptive immunity promoting T helper 2 cells response are activated in the long-term before functional alterations develop. It consequently might trigger the fibrosis promoting processes.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

775-783

Source:

Physiological research | 2019 Volume:68 | Number:5

Harvested from:

CDK

Metadata only:

false