Suramin Affects Capsaicin Responses and Capsaicin-Noxious Heat Interactions in Rat Dorsal Root Ganglia Neurones

Title:

Suramin Affects Capsaicin Responses and Capsaicin-Noxious Heat Interactions in Rat Dorsal Root Ganglia Neurones

Creator:

Viktorie Vlachová, Alla Lyfenko, Ladislav Vyklický, and Orkand, R. K.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2c279a48-df41-4cf4-aba7-32bd1bc9a9f4
uuid:2c279a48-df41-4cf4-aba7-32bd1bc9a9f4
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Dorsal root ganglia neurones, Vanilloid receptor, Capsaicin, Noxious heat, Whole-cell, 14, and 612

Type:

article, studie, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The effect of suramin, an inhibitor of G protein regulated signalling, was studied on the membrane currents induced by noxious heat and by capsaicin in cultured dorsal root ganglia neurones isolated from neonatal rats. Whole-cell responses induced by a heat ramp (24-52 °C) were little affected by suramin. The noxious heat-activated currents were synergistically facilitated in the presence of 0.3 µM capsaicin 13.2-fold and 6.3-fold at 40 °C and 50 °C, respectively. In 65% of neurones, the capsaicin-induced facilitation was inhibited by 10 µM suramin to 35±6 % and 53±6 % of control at 40 °C and 50 °C (S.E.M., n=15). Suramin 30 µM caused a significant increase in the membrane current produced by a nearly maximal dose (1 µM) of capsaicin over the whole recorded temperature range (2.4-fold at 25 °C and 1.2-fold at 48 °C). The results demonstrate that suramin differentially affects the interaction between capsaicin and noxious heat in DRG neurones and thus suggest that distinct transduction pathways may participate in vanilloid receptor activation mechanisms., V. Vlachová, A. Lyfenko, L. Vyklický, † R.K. Orkand., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2002 Volume:51 | Number:2

Harvested from:

CDK

Metadata only:

false