Characterization of 16 novel microsatellite loci for Ephippiger diurnus (Orthoptera: Tettigoniidae) using pyrosequencing technology and cross-species amplification

Title:

Characterization of 16 novel microsatellite loci for Ephippiger diurnus (Orthoptera: Tettigoniidae) using pyrosequencing technology and cross-species amplification

Creator:

Esquer-Garriogos, Greenfield, Michael D., Party, Virginie, and Streiff, Réjane

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:2562946d-cb81-4e68-9c9f-edea970761a5
uuid:2562946d-cb81-4e68-9c9f-edea970761a5
issn:1210-5759
doi:10.14411/eje.2016.037

Subject:

Zoologie, rovnokřídlí, kobylkovití, populační genetika, Orthoptera, Tettigoniidae, population genetics, Ephippiger diurnus, calling song, microsatellite polymorphism, katydid, 2, and 59

Type:

article, články, journal articles, model:article, and TEXT

Format:

print, počítač, and online zdroj

Description:

A novel panel of 16 microsatellite markers, obtained by pyrosequencing of enriched genomic libraries, is reported for the flightless European bushcricket Ephippiger diurnus (Dufour) (Orthoptera: Tettigoniidae). Five multiplex and one simplex PCR protocols were optimized, and the polymorphism at the 16 loci was assessed in two natural populations from southern France. The mean allele number and (expected mean heterozygosity) were 8.94 (0.71) and 6.57 (0.70), respectively, in each population. Several loci were at Hardy-Weinberg disequilibrium (HWD), possibly due to the incidence of null alleles. The occurrence of null alleles has been previously reported for this species, and it is a common feature of microsatellite loci in Orthoptera. Cross-amplification tests demonstrated the transferability of some of these loci to other ephippigerine species. The microsatellite loci reported here substantially increase the number of available loci for this species and will afford an accurate picture of E. diurnus phylogeography, the genetic structure of its populations, and an improved understanding of the evolution of male song and other sexually-selected traits in this highly variable species., Yareli Esquer-Garrigos, Michael D. Greenfield, Virginie Party, Réjane Streiff., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

European Journal of Entomology | 2016 Volume:113

Harvested from:

CDK

Metadata only:

false