Gas system and method for titration of intact leaves with carbon dioxide

Title:

Gas system and method for titration of intact leaves with carbon dioxide

Creator:

Oja, V. and Laisk, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:24ad361b-facb-4443-9101-8589443cdc2d
uuid:24ad361b-facb-4443-9101-8589443cdc2d
urnnbn:urn:nbn:cz:aba007-000fmr

Subject:

boundary layer resistance, buffer capacity of chloroplasts, carbonic anhydrase, CO2 concentration, CO2 evolution, Helianthus annuus L., leaf chamber, pH effect on photosynthesis, pH measurement, and sunflower

Type:

model:internalpart and TEXT

Format:

print

Description:

Carbon dioxide, a natural acidic gas, may be ušed for in vivo titration of the buffers in the compartments of cells that contain carbonic anhydíase in intact leaves. A gas systém for the measurement of the CO2 capacity (total inorganic carbon content) of leaves under different extemal CO2 concentrations has been developed. The systém consists of a leaf chamber and of two open (flow-through) channels, one for pre- loading the leaf with CO2 and the other for measurement of the desolubilized CO2. CO2 concentration in the loading channel may be adjusted from 0.03 to 20 %, while CO2 free air is flowing in the burst (desolubilization) channel. The leaf chamber (4.3 X 4.3 X 0.3 cm^) is switched either into the loading channel or into the burst channel. After loading the leaf with high CO2 in the dark the .chamber is switched into the burst channel and the desolubilized CO2 is measured. Preliminary measurements show that the CO2 capacity of a sunflower leaf decreases when the CO2 concentration is increased from 0.03 to 15 %, because of saturation of buffers. The systém can be ušed for direct, non-destructive measurements of pH and buffer capacity in leaf chloroplasts in order to investigate the influence of pH on photosynthesis, the operation of proton pumps and other pH-stabilising mechanisms.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

37-50 and 39-52

Source:

Photosynthetica | 1995 Volume:31 | Number:1

Harvested from:

CDK

Metadata only:

false