Idebenone-induced recovery of glycerol-3-phosphate and succinate oxidation inhibited by digitonin

Title:

Idebenone-induced recovery of glycerol-3-phosphate and succinate oxidation inhibited by digitonin

Creator:

Hana Rauchová, Martina Vokurková, and Zdeněk Drahota

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:239826b4-9a5b-45c1-bf6e-17481bc55265
uuid:239826b4-9a5b-45c1-bf6e-17481bc55265

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, hyperthyroid liver mitochondria, oxygen consumption rate, coenzyme Q, cytochrome c, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Digitonin solubilizes mitochondrial membrane, breaks the integrity of the respiratory chain and releases two mobile redoxactive components: coenzyme Q (CoQ) and cytochrome c (cyt c). In the present study we report the inhibition of glycerol-3- phosphate- and succinate-dependent oxygen consumption rates by digitonin treatment. Our results show that the inhibition of oxygen consumption rates is recovered by the addition of exogenous synthetic analog of CoQ idebenone (hydroxydecylubiquinone; IDB) and cyt c. Glycerol-3-phosphate oxidation rate is recovered to 148 % of control values, whereas succinatedependent oxidation rate only to 68 %. We find a similar effect on the activities of glycerol-3-phosphate and succinate cytochrome c oxidoreductase. Our results also indicate that succinate-dependent oxidation is less sensitive to digitonin treatment and less activated by IDB in comparison with glycerol- 3-phosphate-dependent oxidation. These findings might indicate the different mechanism of the electron transfer from two flavoprotein-dependent dehydrogenases (glycerol-3-phosphate dehydrogenase and succinate dehydrogenase) localized on the outer and inner face of the inner mitochondrial membrane, respectively., H. Rauchová, M. Vokurková, Z. Drahota., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:3

Harvested from:

CDK

Metadata only:

false