Chronic effects of somatotropin treatment in Vivo and in Vitro on lipogenic activity of goat adipose tissue in a glucose-free buffer during acute incubation

Title:

Chronic effects of somatotropin treatment in Vivo and in Vitro on lipogenic activity of goat adipose tissue in a glucose-free buffer during acute incubation

Creator:

Josef Škarda

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1b9847e1-22c1-41d9-af26-0ba84cf63619
uuid:1b9847e1-22c1-41d9-af26-0ba84cf63619
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, kozy, tuková tkáň, kortizol, goats, adipose tissue, cortisol, somatotropin, lipogenesis, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Young castrated male goats (n = 8) were used to investigate the effect of long-term treatment with recombinant methionyl bovine somatotropin in a sustained release vehicle (bST; 100 mg at seven-day intervals in a 147-day experiment) and chronic culture (24 h) of omental adipose tissue in the presence of various hormones on lipogenic responses to catecholamines during acute incubation (2 h) in a sodium acetate supplemented glucose-free buffer. The rate of fatty acid synthesis in freshly-prepared adipose explants was low and did not differ from those cultured in the absence of hormones for 24 h. Hormonal combination of insulin (17 nmol.l-1) plus cortisol (138 nmol.l-1) or insulin plus recombinant enterokinase linker bST (4.5 nmol.l-1) increased lipogenesis (P<0.05). Further addition of bST or cortisol decreased lipogenesis significantly (P<0.05) in the controls but not significantly in bST-treated animals. Cultured explants from either control or bST-treated animals showed significant inhibition of lipogenesis by both norepinephrine (10 m mol.l-1) and isoprenaline (10 m mol.l-1). BST treatment in vivo did not increase the responsiveness of cultured explants to norepinephrine in vitro, however, the responsiveness to isoprenaline(inhibition of lipogenesis) was greater in bST-treated animals than in the controls., J. Škarda., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1999 Volume:48 | Number:1

Harvested from:

CDK

Metadata only:

false