Allergic sensitization increases contractile responses to 5-HT in guinea pig aorta

Title:

Allergic sensitization increases contractile responses to 5-HT in guinea pig aorta

Creator:

Campos-Bedolla, Patricia, De-La-Cruz-Negrete, Rafael, Vargas, Mario Humberto, Torrejón-González, Emmanuel Gilberto, Mejía-Mendoza, Dinora, Islas-Hernández, Azul, Segura-Medina, Patricia, Córdoba-Rodríguez, Guadalupe, Orozco-Suárez, Sandra, and Arreola-Ramírez, José Luis

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:17cef559-150a-48f9-b629-c9903da928bb
uuid:17cef559-150a-48f9-b629-c9903da928bb
doi:10.33549/physiolres.934128

Subject:

5-HT, serotonin, 5-HT2A receptor, asthma model, vascular responsiveness, thoracic aorta, and vascular smooth muscle

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Epidemiological and clinical studies suggest that asthma is associated with adverse cardiovascular outcomes, but its mechanism is uncertain. 5-Hydroxytryptamine (5-HT) is a mediator involved in asthma and in cardiovascular functioning. Thus, in the present study, we explored whether allergic sensitization in guinea pigs modifies 5-HT-induced contractile responses and 5-HT2A receptor expression in thoracic aorta rings. We found that sensitization produced a significant increase of 100 µM 5-HT-induced contractions of aorta rings (~27 % greater contraction than in non-sensitized animals, p<0.05). Preincubation with 10 nM ketanserin (a 5-HT2A receptor antagonist) reduced by ~30 % (p=0.003) and ~36 % (p=0.005) the area under the curve of 5-HT-induced contractions in aortas from non-sensitized and sensitized animals, respectively. There were no differences between sensitized and non-sensitized animals with respect to mRNA (qPCR) and protein (Western blot) expression of 5-HT2A receptor in thoracic aortas. We concluded that in this guinea pig model of asthma, allergic sensitization is not confined to airways, but also affects arterial contractile responses to 5-HT; changes in the expression of the 5-HT2A receptor appear not to be involved in this phenomenon.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

191-197

Source:

Physiological research | 2020 Volume:69 | Number:1

Harvested from:

CDK

Metadata only:

false