Overexpression of calmodulin gene fragment from Antarctic notothenioid fish improves chilling tolerance in Nicotiana benthamiana

Title:

Overexpression of calmodulin gene fragment from Antarctic notothenioid fish improves chilling tolerance in Nicotiana benthamiana

Creator:

Zhang, T. J., Pan, L. J., Huang, Qingrong, Zhu, L. H., Yang, N., Peng, C. L., and Chen, L. B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:0cbb9a33-70ee-4e5a-986a-cf1eec1f15ae
uuid:0cbb9a33-70ee-4e5a-986a-cf1eec1f15ae
issn:0300-3604
doi:10.1007/s11099-016-0682-z

Subject:

obecná botanika, general botany, calcium-binding protein, chilling stress, chlorophyll fluorescence, electrolyte leakage, 2, and 581

Type:

model:article and TEXT

Format:

print, bez média, and svazek

Description:

Calmodulin (CaM) is a highly conserved calcium sensor protein associated with chilling tolerance in living organisms. It has four EF-hand domains for binding of four Ca2+, two of them located in the N-terminus, and the other two in the C-terminus. A notothenioid CaM gene fragment (CaMm), which only codes for N-terminus of CaM (with two EF-hand domains), was introduced into Nicotiana benthamiana. Effects of its overexpression on chilling tolerance in plants were explored. During 4◦C or 0◦C chilling treatment, both CaMm and CaM transgenic plants showed higher PSII maximum quantum yield, actual quantum yield, and soluble protein content, lower electrolyte leakage and malondialdehyde content than that of the control. The changes in these physiological indices were comparable between the CaMm and CaM transgenic plants during the treatments. These results indicate that the N-terminus of calmodulin is likely the key functional domain involved in the adaptive response to cold stress., T. J. Zhang, L. J. Pan, Q. Huang, L. H. Zhu, N. Yang, C. L. Peng, L. B. Chen., and Obsahuje seznam literatury

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

630-637

Source:

Photosynthetica | 2017 Volume:55 | Number:4

Harvested from:

CDK

Metadata only:

false