Glutathione reductase is inhibited by acetaminophen-glutathione conjugate in vitro

Title:

Glutathione reductase is inhibited by acetaminophen-glutathione conjugate in vitro

Creator:

Tomáš Roušar, Patrik Pařík, Otto Kučera, Bartoš, M., and Zuzana Červinková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:070db48c-88e5-41e8-82ca-4c36336b81ba
uuid:070db48c-88e5-41e8-82ca-4c36336b81ba

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, acetaminophen toxicity, glutathione reductase, glutathione, hepatotoxicity, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

The aim of the present work was to investigate a new mechanism likely contributing to the toxic action of acetaminophen, especially to explore the possible inhibition of glutathione reductase through an acetaminophen-glutathione conjugate (APAP-SG). APAP-SG conjugate was synthesized by organic synthesis and purified by column chromatography. The inhibitory effect of the conjugate on two types of glutathione reductase (from yeasts and rat hepatocytes) was tested spectrophotometrically. We found that the enzyme activity was reduced similarly after the treatment with 2.96 mM acetaminophenglutathione conjugate in both yeast and hepatocyte glutathione reductases (GR); the enzyme activity was inhibited to 52.7±1.5 % (2.4±0.3 mU/ml) in yeast GR (control activity was 5.6±0.3 mU/ml) and to 48.1±8.8 % (2.2±0.2 mU/ml) in rat hepatocytes lysate GR (control activity was 5.2±0.2 mU/ml). In addition, the enzyme activity (from hepatocytes lysate) was decreased to 79±7 %, 67±2 % and 39±7 %, in 0.37, 1.48 and 3.7 mM concentration of the conjugate, respectively. We found that glutathione reductase, the essential enzyme of the antioxidant system, was dose-dependently inhibited by the product of acetaminophen metabolism - the conjugate of acetaminophen and glutathione., T. Roušar ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2010 Volume:59 | Number:2

Harvested from:

CDK

Metadata only:

false