We investigated the physiological adaptations for winter survival in a freeze-intolerant chrysomelid, Aulacophora nigripennis, in warm-temperate regions. The adults showed a decreased supercooling point (SCP), increased chill tolerance and high myo-inositol content during winter. Chill tolerance at 0°C appears to be a more suitable indicator of their cold hardiness than SCP because they die at 0°C without freezing and normally an not exposed to subzero temperatures below their SCP., Masahiko Watanabe, Kazuhiro Tanaka, and Lit