An entirely new method of optical microscopy in transmitted light “Relief Contrast after Hostounsk ̆” or RCH, Lambda Ltd. Praha, Czech Republic, was used to study of integument surface replicas of reptiles (microrelief adhesive method after Wolf) as well as reptilian sloughts. This equipment provides a three-dimensional image of high contrast and resolution. Compared to microscopy without phase or interference contrast, RCH-microscopy makes it possible to evaluate a three-dimensional organization of microrelief on reptilian scales. Results obtained from these microscopical observations can be used for both ecological and taxonomical studies on animals.