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Start Over Subject microsporidium

1. Occurrence of a new microsporidium in the skeletal muscle of the flying fish Cypselurus pinnatibarbatus japonicus (Exocoetidae) from Yakushima, Japan

Creator:
Yokoyama, Hiroshi, Lee, Sun-Joung, and Bell, Andrew S.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Microspora, microsporidium, flying fish, and Cypselurus pinnatibarbatus
Language:
English
Description:
A new microsporidium was observed in the flying fish Cypselurus pinnatibarbatus japonicus (Franz) (Exocoetidae) from Yakushima, Japan. Visual examination revealed the microsporidium to form white elongate nodules in the host's trunk muscle. Monomorphic spores were ovoid to pyriform in shape, with average dimensions of 4.1 × 2.2 µm and possessing a polar tube describing 13-15 coils. Histological observations showed that each parasite focus of infection was encapsulated by a host-produced fibrous membrane. The presence of sporophorous vesicles was not clearly determined. Ribosomal DNA sequence analyses showed the microsporidium to be discrete from other known fish muscle-infecting species and to be most closely related to a clade comprising the Pleistophoridae and Glugea spp. The parasite is provisionally placed as Microsporidium cypselurus sp. n.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Phylogenetic characterization of a microsporidium (Nosema sp.) isolated from the mulberry pest, Hemerophila atrilineata

Creator:
Guan, Rui, Shen, Zhongyuan, Zhu, Feng, Chen, Darui, Zhang, Jiao, Hou, Jiange, Dong, Shinan, Tang, Xudong, and Xu, Li
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Nosema sp. HA, gene organization, microsporidium, rRNA gene, and phylogenetic analysis
Language:
English
Description:
Microsporidia are a group of obligate intracellular unicellular eukaryotes that can parasitize a wide variety of other eukaryotes ranging from protists to invertebrates and vertebrates. In this study, we examined the microsporidium Nosema sp. isolated from the mulberry pest, Hemerophila atrilineata Butler, 1881, named herein ''Nosema sp. HA''. The fresh spores were long oval in shape, 3.8 ± 0.4 μm in length and 1.9 ± 0.3 μm in width. Analysis of tissue infection of silkworm, Bombyx mori Linnaeus, 1758, indicated that the midgut, Malpighian tubules, muscle, fat body, silk glands, hemocytes, nerve tissue and gonads of silkworm were infected with Nosema sp. HA. The complete rRNA gene sequence of this microsporidium contained 4 305 base pairs (GenBank Accession JN882299), including the large subunit rRNA (2 492 bp), the internal transcribed spacer (187 bp), the small subunit rRNA (1 232 bp), the intergenic spacer (279 bp) and the 5S region (115 bp). The organization of the rRNA gene is 5'-LSU-ITS-SSU-IGS-5S-3'. Phylogenetic analysis, comparison of sequence identities and the arrangement in the rRNA gene subunits suggested that this isolate is separate from other Nosema species.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public