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2. T-lymphopoiesis is severely compromised in ubiquitin-green fluorescent protein transgenic mice

Creator:
Faltusová, K. , Báječný, M., Heizer, T., Páral, P., and Nečas, E.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
UBC-GFP mice, C57Bl, J mice, lymphopoiesis, myelopoiesis, haematopoiesis, stem cell, transplantation, green fluorescent protein, T cell, and B cell
Language:
English
Description:
Tagging cells of experimental organisms with genetic markers is commonly used in biomedical research. Insertion of artificial gene constructs can be highly beneficial for research as long as this tagging is functionally neutral and does not alter the tissue function. The transgenic UBC-GFP mouse has been recently found to be questionable in this respect, due to a latent stem cell defect compromising its lymphopoiesis and significantly influencing the results of competitive transplantation assays. In this study, we show that the stem cell defect present in UBC-GFP mice negatively affects T-lymphopoiesis significantly more than B-lymphopoiesis. The production of granulocytes is not negatively affected. The defect in T-lymphopoiesis causes a low total number of white blood cells in the peripheral blood of UBC-GFP mice which, together with the lower lymphoid/myeloid ratio in nucleated blood cells, is the only abnormal phenotype in untreated UBCGFP mice to have been found to date. The defective lymphopoiesis in UBC-GFP mice can be repaired by transplantation of congenic wild-type bone marrow cells, which then compensate for the insufficient production of T cells. Interestingly, the wild-type branch of haematopoiesis in chimaeric UBC-GFP/wild-type mice was more active in lymphopoiesis, and particularly towards production of T cells, compared to the lymphopoiesis in normal wild-type donors.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

3. Trafficking of Plasmodium falciparum chimeric rhoptry protein with Brefeldin A

Creator:
Ghoneim, Ahmed M.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
green fluorescent protein, apical secretions, targeting, organelles, merozoite, and Apicomplexa
Language:
English
Description:
Trafficking of the rhoptry chimeric protein RhopH2-GFP, which contains RhopH2 signal peptide plus the downstream five amino acids, was dissected by treating parasites with Brefeldin A at three different time points. Twenty eight hrs-stage trophozoites accumulated the chimera within the parasite endoplasmic reticulum. In 32 hrs-stage schizonts, the chimera was distributed in the parasite cytoplasm but not in the parasitophorous vacuole. In 36 hrs stage-schizonts, the chimera was detected in the individual parasitophorous vacuoles of the developing merozoites and, in contrary to non-treated parasites, no immature rhoptry vesicles could be detected in the cytoplasm of immature merozoites. These data show that this chimera is trafficked to the rhoptries via Brefeldin A-sensitive pathway indicating that this trafficking is similar to that of the endogenous rhoptry proteins, and that the five amino acids downstream of the signal peptide cleavage site may contain the sorting signal required for rhoptry targeting.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public