IsiA is a membrane-bound Chl a-antenna protein synthesized in cyanobacteria under iron deficiency. Since iron deficiency is a common nutrient stress in significant fractions of cyanobacterial habitats, IsiA is likely to be essential for some cyanobacteria. However, the role it plays in cyanobacteria is not fully understood. In this review paper, we summarize the research efforts directed towards characterizing IsiA over the past three decades and attempt to bring all the pieces of the puzzle together to get a more comprehensive understanding of the function of this protein. Moreover, we analyzed the genomes of over 390 cyanobacterial strains available in the JGI/IMG database to assess the distribution of IsiA across the cyanobacterial kingdom. Our study revealed that only 125 such strains have an IsiA homolog, suggesting that the presence of this protein is a niche specific requirement, and cyanobacterial strains that lack IsiA might have developed other mechanisms to survive iron deficiency., H.-Y. S. Chen, A. Bandyopadhyay, H. B. Pakrasi., and Obsahuje bibliografické odkazy
Previously, our data indicated that both cAMP and MAP kinase signaling play important roles in microalgal physiology as well as in lipid or carotenoid biosynthesis. In order to understand downstream genes of these signaling pathways, we employed proteomics approach. Both signal pathways were first altered with specific signaling inhibitors or modulators. Treatment of specific inhibitors changed microalgal size and increased lipid contents. With the microalgal cells after treatments of specific signaling inhibitor or modulators, we performed the proteomics analysis to identify downstream genes responsible for these phenotypes. Interestingly, multiple photosynthesis genes were identified, particularly proteins associated with PSII. Our data suggested that MAP kinase and cAMP signaling affect the photosynthesis, thereby leading to microalgal lipid or carotenoid biosynthesis., C. Lee, J.-K. Rhee, D. G. Kim, Y.-E. Choi., and Obsahuje seznam literatury