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Start Over Subject elektroforéza

1. Bezkontaktní vodivostní detektor pro kapilární elektroforézu

Creator:
Kubáň, Pavel
Format:
print, bez média, and svazek
Type:
article, články, journal articles, model:article, and TEXT
Subject:
Věda. Všeobecnosti. Základy vědy a kultury. Vědecká práce, analytická chemie, elektroforéza, detekce (chemie), analytical chemistry, electrophoresis, detection (chemistry), 12, and 00
Language:
Czech
Description:
Pavel Kubáň.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

2. The time-course of agonist-induced solubilization of trimetric Gqα/G11α proteins resolved by two-dimensional electrophoresis

Creator:
Dana Durchánková, Jiří Novotný, and Petr Svoboda
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, bílkoviny, elektroforéza, thyrotr, physiology, proteins, electrophoresis, G proteins, solubilization, Gqα/G11α, thyrotropin-releasing hormone, TRH receptor, 14, and 612
Language:
English
Description:
Prolonged agonist stimulation results in specific transfer of activated Gα subunits of Gqα/G11α family from particulate membrane fraction to soluble (cytosol) cell fraction isolated as 250 000 x g supernatant. In this study, we have used 2D electrophoresis for more defined resolution of Gα subunits of Gqα/G11α family and followed the time course of solubilization effect. The small signal of soluble G proteins was already detected in control, hormone-unexposed cells. Hormone stimulation resulted in a slow but continuous increase of both intensity and number of immunoreactive signals/spots of these G proteins (10, 30, 60, 120 and 240 min). At longer times of agonist exposure (>2 hours), a marked increase of Gqα/G11α proteins was detected. The maximal level of soluble Gqα/G11α proteins was reached after 16 hours of continuous agonist exposure. At this time interval, eight individual immunoreactive signals of Gqα/G1 α proteins could be resolved. The relative proportion among these spots was 15:42:10:11:7:7:2:5. Solubilization of this class of Gα proteins was thus observed after prolonged agonist stimulation only, induced by ultra high concentration of hormone and in cells expressing a large number of GPCRs. Our data therefore rather indicate tight/persisting binding of Gqα/G11α proteins to the membrane., D. Durchánková, J. Novotný, P. Svoboda., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Zdvojené paraproteinémie - krátké sdělení

Creator:
Tichý, Miloš, Vávrová, Jaroslava, and Maisnar, Vladimír
Type:
model:article, article, Text, and TEXT
Subject:
lidé, financování organizované, paraproteinémie--imunologie--klasifikace, mnohočetný myelom, klinické laboratorní techniky--trendy--využití, imunoglobuliny--diagnostické užití--izolace a purifikace--klasifikace, biklonální gamapatie, elektroforéza, and imunofixace
Language:
Czech and English
Description:
Pro ověření frekvence výskytu zdvojených paraproteinémií jsme analyzovali soubor 500 paraproteinémií, které jsme nově prokázali během 46 měsíců. Zdvojenou paraproteinémii, tj. takovou, u níž se paraproteiny liší typem lehkých řetězců, třídou těžkých řetězců imunoglobulinů nebo oběma těmito znaky, jsme prokázali celkem ve 27 pozorováních, tj. v 5,4% frekvenci. Z klinického hlediska šlo v 52 % o BGUS (biclonal gammopathy of undetermined significance), v 18 % o mnohočetný myelom a ve 30 % o jiné lymfoproliferativní onemocnění., We analyzed group of 500 paraproteinemias for presence double paraproteinemias, which were newly proved in our laboratory during 46 months. Double paraproteinemias were confirmed in 27x (5.4 %) cases. They were in 52 % patients with BGUS (biclonal gammopathy of undetermined significance), in 18 % with multiple myeloma and in 30 % with other lymphoproliferative diseases., Tichý M., Vávrová J., Maisnar V., and Lit.: 9
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public