The aim of this study is to detect changes in flow regime of rivers in Poland. On the basis of daily discharges recorded in 1951–2010 at 159 gauging stations located on 94 rivers regularities in the variability of the river flow characteristics in the multi-year period and in the annual cycle were identified and also their spatial uniformity was examined. In order to identify changes in the characteristics of river regime, similarities of empirical distribution functions of the 5-day sets (pentads) of discharges were analyzed and the percent shares of similar and dissimilar distributions of the 5-day discharge frequencies in the successive 20-year sub-periods were compared with the average values of discharges recorded in 1951–2010. Three alternative methods of river classification were employed and in the classification procedure use was made of the Ward’s hierarchical clustering method. This resulted in identification of groups of rivers different in terms of the degree of transformation of their hydrological regimes in the multi-year and annual patterns.