Studies were conducted to investigate the distribution of larvae of the European vine moth, Lobesia botrana (Denis & Schiffermüller) (Lepidoptera: Tortricidae), a key vineyard pest of grape cultivars. The data collected were larval densities of the second and third generation of L. botrana on half-vine and entire plants of wine and table cultivars in 2003-2004. No insecticide treatments were applied to plants during the 2-year study. The distribution of L. botrana larvae can be described by a negative binomial. This reveals that the insect aggregates. A common value for the k parameter of the negative binomial distribution of kc = 0.6042, was obtained, using maximum likelihood estimation, and the advantages and cases of use of a common k are discussed. The k-1Sinh-1(ksqrt{x+1/2}) and k-1Sinh-1(ksqrt{x+3/8}) proved to be the best transformations for L. botrana larval counts. An entire vine is recommended as the sampling unit for research purposes, whereas a half-vine, which is suitable for grape vine cultivation in northern Greece, is recommended for practical purposes. We used these findings to develop a fixed precision sequential sampling plan and a sequential sampling program for classifying the pest status of L. botrana larvae.