The major light-harvesting chlorophyll (Chl) a/b complexes of photosystem II (LHCIIb) play important roles in energy balance of thylakoid membrane. They harvest solar energy, transfer the energy to the reaction center under normal light condition and dissipate excess excitation energy under strong light condition. Many bamboo species could grow very fast even under extremely changing light conditions. In order to explain whether LHCIIb in bamboo contributes to this specific characteristic, the spectroscopic features, the capacity of forming homotrimers and structural stabilities of different isoforms (Lhcb1-3) were investigated. The apoproteins of the three isoforms of LHCIIb in bamboo are overexpressed in vitro and successfully refolded with thylakoid pigments. The sequences of Lhcb1 and Lhcb2 are similar and they are capable of forming homotrimer, while Lhcb3 lacks 10 residues in the N terminus and can not form the homotrimeric structure. The pigment stoichiometries, spectroscopic characteristics, thermo- and photostabilities of different reconstituted Lhcbs reveal that Lhcb3 differs strongly from Lhcb1 and Lhcb2. Lhcb3 possesses the lowest Qy transition energy and the highest thermostability. Lhcb2 is the most stable monomer under strong illumination among all the isoforms. These results suggest that in spite of small differences, different Lhcb isoforms in bamboo possess similar characteristics as those in other higher plants., Z. H. Jiang ... [et al.]., and Obsahuje bibliografii
The first detailed description of larvae of Ptecticus Loew is presented for P. brunettii and P. flavifemoratus from West Malaysia. There are pronounced morphological and behavioural differences between the last larval instar, inside whose cast cuticle the pupa remains, and earlier instars. The larval mouthparts are similar to those of other known Stratiomyidae larvae but may display a set of autapomorphic characters. The structure of the mandibular-maxillary complex suggests that larvae of Ptecticus are micropantophagous scavengers that feed chiefly upon microorganisms. The larvae of both species are associated with decaying bamboo shoots; P. brunettii inhabits the space between the culm sheaths and P. flavifemoratus lives in water-filled shoot stumps. This is the first record of aquatic Sarginae larvae and egg plastron in the Stratiomyidae. The life cycle and behaviour of P. brunettii and P. flavifemoratus is described and the resource partitioning of stratiomyids and xylomyids associated with bamboo is discussed.
Amphicrossus japonicus is the first known facultatively aquatic nitidulid. The adult beetles breed in bamboo sap and subsequently enter water-filled bamboo culms. In water they breathe via a ventral air sheath held by hydrofuge pubescence. The beetles are facultative predators and hunt mosquito larvae, which they grab with their forelegs. The trend to facultative predation in Cucujoidea and the transition for beetles in general from semi-liquid decaying organic matter into water is discussed.