Five new records of the scale-bearing chrysophytes Mallomonas multiunca, M. paxillata, M. portae-ferreae, M. prora and M. retifera are reported for the Czech Republic. M. multiunca was found in a mesotrophic oxbow lake of the river Vltava. M. paxillata, M. portae-ferreae and M. prora were found in a mesotrophic to eutrophic floodplain pool of the river Lužnice. M. retifera was recorded from alluvial pools associated with the Vltava and Lužnice rivers, respectively. Autecology and distribution of the species are discussed. Three of the species occur in temperate and subarctic regions, M. paxillata probably has a cosmopolitan distribution and M. portae-ferreae prefers warmer water, occurring most frequently in tropical to subtropical regions.
In Cobitis taenia and, with lower intensity, also in C. elongatoides and C. melanoleuca, females bear visible spawning marks on the body sides after spawning events. These marks intensify during the spawning season but disappear a few weeks after the last spawning event. The precise origin of the spawning marks is not clear, but they seem to be produced by the male during embracement of the female. Spawning marks were not observed after spawning events of C. bilineata, C. fahirae, C. rara, C. paludica, C. strumicae and C. turcica. An example dataset from Haaren creek in northwestern Germany is given to demonstrate that these marks can be used as a tool to precisely estimate the start of reproductive season for Cobitis populations, separately even for different size classes.