The blood-sucking bug Rhodnius prolixus is the main vector of Chagas Disease in Colombia, Venezuela and several countries in Central America. Nitric oxide (NO) is a ubiquitous gaseous molecule present in most types of cell and participates in the olfactory pathway of insects. In this work, nitroso-acetyl-cysteine (SNAC), a nitric oxide donor, was topically applied to the antennae of fifth instar nymphs of R. prolixus. After SNAC treatment, these insects showed a dose-dependent reluctance to feed when provided with a living pigeon as the food source (ED50 = 5.2 µg/insect). However, there was no reluctance to feed when db-cGMP was applied to the antennae of nymphs. In another experiment, insects that had their antennae treated with SNAC were less attracted than the control group to a CO2 source. A possible role of NO in the olfactory pathway of R. prolixus is discussed. and Valeria Sfara, Eduardo N. Zerba, Raúl A. Alzogaray.