During a survey of parasites of Percichthys trucha (Cuvier et Valenciennes) (Perciformes: Percichthyidae) in northwestern Patagonian lakes, a new dracunculoid of the family Guyanemidae was found parasitizing as adults the heart of the fish. This species belongs to the genus Pseudodelphis Adamson et Roth, 1990 by having deirids, glandular multinucleate oesophagus and anterior branch of the uterus, and by lacking caudal alae and a gubernaculum. Pseudodelphis limnicola sp. n. differs from Pseudodelphis oligocotti Adamson et Roth, 1990 by the number and distribution of the cephalic papillae, a muscular oesophagus submerged into the glandular portion and by lacking a peribuccal ring. This is the first record of a guyanemid inhabiting the heart of a Neotropical perciform fish.