The chromosome numbers of 95 populations of taxa belonging to the Scilla bifolia group growing in the territory of Western Carpathians, and adjacent part of the Pannonian lowland (Slovakia, Hungary, Austria, Czech Republic – Moravia) are presented. Scilla kladnii Schur (41 populations) and S. vindobonensis Speta (18 populations) have only the chromosome number 2n = 18. Both these diploid taxa seem to be karyologically uniform throughout their distribution. For S. drunensis (Speta) Speta subsp. drunensis (2 populations), and S. drunensis subsp. buekkensis (Speta) Kereszty (15 populations) the chromosome number 2n = 36 was found. For S. spetana Kereszty (2 populations), the chromosome number 2n = 54 was confirmed for Hungary and Austria. For two groups of populations of S. drunensis s.l. from Slovakia (14 populations in total) the chromosome number is 2n = 36, which is new information for the Western Carpathians. Apart from tetraploids, hexaploid populations with 2n = 54 were confirmed for three localities in Slovakia and Czech Republic (Moravia). All results are compared with earlier published data.