A total of 39 Amiota species are found from the southern portion of Hengduan Mountains, southwestern China, including 12 new species: A. gaoi sp. n., A. gracilenta sp. n., A. multispinata sp. n., A. yifengi sp. n., A. angustifolia sp. n., A. bacillia sp. n., A. biacuta sp. n., A. cultella sp. n., A. deltoidea sp. n., A. pianmensis sp. n., A. setosa sp. n. and A. bifoliolata sp. n. A key to all the studied species from Hengduan Mountains is provided.