The author analyzes the influence of the EU enlargement on women's and feminist NGOs and women's groups in the Czech Republic. She identifies main proceeding changes in non-governmental sphere and civic mobilization which are analyzed as consequences of Czech Republic's accession to the EU. Namely she discuss processes of professionalization - hence establishment of professional women's and feminist NGOs and development of political lobby; processes of developing of project-orientation - it reflects the necessity to apply for grants largely in EU to finance operation of organizations. Finally she identifies processes of acceptation of reform-orientation of women's NGOs in the Czech Republic - which means orientation on mainstream issues that are encouraged by EU policies. As a result of these changes some small and marginal NGOs disappeared and others were determined to make strategic partnership across the thematic focus.