Chlorophyllase (Chlase) actívity measured as the in vitro degradation of chlorophyll (Chl) was variably enhanced by various heavy metals (Cd2+, Pb2+, Mn2+, Co^"*", Ni2+ and their mixture). The en2yme actívity on Chl a was higher than that on Chl b. Chlase isolated from Chlorella fusca oř Kirchneriella lunaris responded differently to the heavy metals. The inclusion of Ca2+ in the assay media affected the enzyme actívity. While some concentrations were stimulatory, others were inhibitory depending on partícular heavy metal, the algal species, and the Chl a oř b.
We investigated the effect of moderate Cu2+ and Cd2+ stress by applying chlorophyll (Chl) fluorescence and P700 absorbance measurements to monitor the photosynthetic electron transport activity of 3-week-old Pisum sativum L. cv. Petit Provençal plants grown in a modified Hoagland solution containing 50 μM CuSO4 or 5 μM CdCl2. Both heavy metals caused a slight inhibition in PSII photochemistry as indicated by the decrease in the effective quantum efficiency of PSII (ΦPSII), the maximum electron transport capacity (ETRmax), and the maximum quantum yield for electron transport (α). PSI photochemistry was also affected by these heavy metals. Cu2+ and Cd2+ decreased the quantum efficiency of PSI (ΦPSI) as well as the number of electrons in the intersystem chain, and the Cu2+ treatment significantly reduced the number of electrons from stromal donors available for PSI. These results indicate that PSII and PSI photochemistry of pea plants are both sensitive to moderate Cu2+ and Cd2+ stress, which in turn is easily detected and monitored by Chl fluorescence and P700 absorbance measurements. Therefore, monitoring the photochemistry of pea plants with these noninvasive, yet sensitive techniques offers a promising strategy to study heavy metal toxicity in the environment., B. Wodala ... [et al.]., and Obsahuje bibliografii