Species of the Agrilus viridis-complex and the genus Trachys are morphologically difficult to identify or even indistinguishable. However, all of them are ecologically clearly separated because their larvae develop in different host plants. Hitherto, it was unclear whether they represent varieties, ecological races or true species. In this paper the genetic variation and phylogenetic relationships within these groups are analysed using partial sequence data from mitochondrial genes (12S rDNA, and a fragment containing regions of ND1 and 16S rDNA). The phylogenetic analyses yielded largely congruent tree topologies and indicate that all species and varieties of the Agrilus viridis-complex belong to a monophyletic group, which is closely related to A. cuprescens. Compared to all other Agrilus-species tested, the genetic distances within the A. viridis-complex are very small. However, all varieties and species are clearly separated. Thus, our data support the view that both the members of the Agrilus viridis-complex and the species of the Trachys-group represent genetically separated taxa.