The genus Agnippe Chambers, 1872 in the Palaearctic Region is revised. Sixteen species are recognized as valid. Three new synonyms are established: A. conjugella Caradja, 1920 = A. haberlandii Amsel, 1961 syn. n., A. lunaki Rebel, 1941 = A. penicillata Amsel, 1961 syn. n., A. kuznetzovi Lvovsky & Piskunov, 1989 = A. zhengi Li, 1993 syn. n. Four new species are described: A. echinuloides sp. n. (S Ural, Tuva, Transbaikalia, Mongolia), A. turanica sp. n. (Uzbekistan, Kazakhstan), A. deserta sp. n. (Uzbekistan) and A. separatella sp. n. (S Iran). Description of all the species and an identification key are presented, accompanied by illustrations of the adults, and male and female genitalia.