The 14-3-3 proteins are a family of acidicr egulatory molecules found in all eukaryotes. 14-3-3 proteins function as molecular scaffolds by modulating the conformation of their binding partners. Through the functional modulation of a wide range of binding partners, 14-3-3 proteins are involved in many processes including cell cycle regulation, metabolism control, apoptosis, and control of gene transcription. This minireview includes a short overview of 14-3-3 proteins and then focuses on their role in the regulation of two important binding partners: FOXO forkhead transcription factors and an enzyme tyrosine hydroxylase., V. Obšilová, J. Šilhan, E. Bouřa, J. Teisinger, T. Obšil., and Obsahuje bibliografii a bibliografické odkazy
Neutral trehalase 1 (Nth1) from Saccharomyces cerevisiae
catalyzes disaccharide trehalose hydrolysis and helps yeast to
survive adverse conditions, such as heat shock, starvation or
oxidative stress. 14-3-3 proteins, master regulators of hundreds
of partner proteins, participate in many key cellular processes.
Nth1 is activated by phosphorylation followed by 14-3-3 protein
(Bmh) binding. The activation mechanism is also potentiated by
Ca2+ binding within the EF-hand-like motif. This review
summarizes the current knowledge about trehalases and the
molecular and structural basis of Nth1 activation. The crystal
structure of fully active Nth1 bound to 14-3-3 protein provided
the first high-resolution view of a trehalase from a eukaryotic
organism and showed 14-3-3 proteins as structural modulators
and allosteric effectors of multi-domain binding partners.
Many aspects of protein function regulation require specific protein-protein interactions to carry out the exact biochemical and cellular functions. The highly conserved members of the 14-3-3 protein family mediate such interactions and through binding to hundreds of other proteins provide multitude of regulatory functions, thus playing key roles in many cellular processes. The 14-3-3 protein binding can affect the function of the target protein in many ways including the modulation of its enzyme activity, its subcellular localization, its structure and stability, or its molecular interactions. In this minireview, we focus on mechanisms of the 14-3- 3 protein-dependent regulation of three important 14-3-3 binding partners: yeast neutral trehalase Nth1, regulator of G-protein signaling 3 (RGS3), and phosducin., V. Obsilova ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy