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2. Dynamic changes of photosynthetic pigments in soybean callus under high irradiance
- Creator:
- Pandey, D. M., Kim, K.-H., and Yeo, U.-D.
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- carotenoids, chlorophyll, Glycine max, HPLC, photoinhibition, spectrophotometry, violaxanthin, and zeaxanthin
- Language:
- Multiple languages
- Description:
- Dynamic changes of neoxanthin (NEO), violaxanthin (VIO), anteraxanthin (ANT), zeaxanthin (ZEA), chlorophyll (Chl) a, Chl b, α-carotene, β-carotene, and their behaviour under increasing duration of high irradiance (HI) were investigated in the soybean hypocotyl callus culture. The calli were induced on solid (1.1 % agar) MS medium (pH 5.8) supplemented with 4.52 μM 2,4-D, 2.32 μM kinetin, and 3 % sucrose. After 30 d of culture, the green calli were irradiated with "white light" (133W m-2) for 0, 3.5, and 24 h. HPLC profiles were separated on a C18 column. With increasing duration of HI, the content of total carotenoids (Cars) increased, but the ratio of Chl a+b/Cars decreased. With lengthening the duration of HI, there was induction of ZEA. Contents of ANT, α-carotene, and β-carotene remained nearly constant, but ratio of ZEA/Chl a+b increased with lengthening the HI duration. and D. M. Pandey, K. H. Kim, U. D. Yeo.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public