Search

Start Over Creator Trojan, S.

1. Are embryonal neurones used for transplantation "sufficiently immature"?

Creator:
Pokorný, J., Langmeier, M., and Trojan, S.
Type:
article, model:article, and TEXT
Subject:
hippocampus, embryonal neurones, neuronal suspension, and dendritic development
Language:
English
Description:
Live neuronal suspensions, prepared from the hippocampal region of donors aged 20 embryonal days, were observed in the Nomarski Differential Interference Contrast. Many neurones displayed profiles, resembling dendrites or axons and both principal hippocampal neurones (pyramidal and granular cells) were identified. For transplantation studies, donors of a younger embryonal age are thus recommended.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Changes in the Number of Nitrergic Neurons Following Kainic Acid Administration and Repeated Long-term Hypoxia

Creator:
Benešová, P., Langmeier, M., Betka, J., and Trojan, S.
Type:
article, model:article, and TEXT
Subject:
Kainic Acid, Nitric Oxide, Hypoxia, Hyppocampus, and Primaryx auditory cortex
Language:
English
Description:
Using histochemical analysis (NADPH-diaphorase) we have been investigating the influence of intraperitoneal administration of kainic acid (KA), hypoxia and combination of both these factors on neurons of the hippocampus and on the primary auditory cortex (PAC) in male rats of the Wistar strain. Kainic acid was administered to 18-day-old animals, which were exposed to long-lasting repeated hypoxia from the 2nd till the 17th day of age in a hypobaric chamber (for 8 h a day). At the age of 22 or 90 days, the animals were transcardially perfused with 4 % paraformaldehyde under deep thiopental anesthesia. Cryostate sections were stained to identify NADPH-diaphorase positive neurons that were then quantified in the hippocampus, in the dentate gyrus and in the PAC. In 22-day-old animals both hypoxia and KA increased the number of NADPH-diaphorase positive neurons in the hilus, CA1, CA3 areas of the hippocampus and in the PAC. On the contrary, KA given to hypoxic animals lowered the number of NADPH-diaphorase positive neurons in the dentate gyrus. In 90-day-old animals, hypoxia and KA given to both normoxic and hypoxic animals lowered the number of NADPH-diaphorase positive neurons in some areas of the central nervous system.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Long-lasting Changes in the Density of Nitrergic Neurons Following Kainic Acid Administration and Chronic Hypoxia

Creator:
Benešová, P., Langmeier, M., Betka, J., and Trojan, S.
Type:
article, model:article, and TEXT
Subject:
Hypoxia, Kainic acid, Nitric oxide, Hippocampus, and Primary auditory cortex
Language:
English
Description:
Using histochemical analysis (NADPH-diaphorase) we have investigated the influence of intraperitoneal administration of kainic acid (KA), hypoxia and combination of both these factors on neurons of the hippocampus and on the primary auditory cortex (PAC) in male rats of the Wistar strain. Kainic acid was administered to 18-day-old animals, which were exposed to long-lasting repeated hypoxia from the 2nd till the 17th day of age in a hypobaric chamber (for 8 hours a day). At the age of 1 year, the animals were transcardially perfused with 4 % paraformaldehyde under deep thiopental anesthesia. Cryostate sections were stained to identify NADP H-diaphorase positive neurons that were then quantified in CA1 and CA3 areas of the hippocampus, in the dentate gyrus and in the PAC. Both, hypoxia and KA lowered the number of NADPH-diaphorase positive neurons in the hilus, dorsal and ventral blades of the dentate gyrus, CA1 and CA3 areas of the hippocampus. On the contrary, KA given to the hypoxic animals increased the number of NADPH-diaphorase positive neurons in the dorsal blade of the dentate gyrus and PAC.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Mitochondrial dysfunction

Creator:
Jandová, A., Pokorný, J., Kobilková , J., Trojan, S., Nedbalová , M., Dohnalová , A., Čoček, A., Mašata, J., Holaj , R., Turzická , E., Zvolský , P., Dvořáková , M., and Cifra, M.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Mitochondria, cell-mediated immunity, cancer, myocardial infraction, schizophrenia, and LAI assay
Language:
English
Description:
Cell-mediated immunity (CMI) response of healthy humans and cancer (Ca) patients to specific tumor antigen and nonspecific (LDV -- lactate dehydrogenase virus) antigen, and of acute myocardial infarction (AMI) and schizophrenia (Sch) patients to nonspecific antigen was investigated. Large differences of CMI response of healthy humans in comparison with Ca, AMI, Sch patients were found. CMI response to antigens displays transferred information about cells under immune surveillance. LDV disturbs the oxidative energy production system. We assume that CMI response to LDV antigen monitors pathological states of mitochondrial energy production which results in disturbances of electromagnetic activity of living cells.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

5. Protective effect of vitamin E on brain ischaemia during ontogenesis

Creator:
Trojan, S.
Type:
article, model:article, and TEXT
Subject:
vilamin E, brain ischaemia, positive radial acceleration lOxg, and ontogenesis
Language:
English
Description:
Vitamin E in a dose of 150 mg and 300 mg/kg of body weight, administered i.p., has a protective effect - in the course of the whole ontogenesis - against stagnant hypoxia induced in the laboratory rat by positive radial acceleration + lOxg. The favourable influence of vitamin E is relatively greater In older animals.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public