The number of little owls, Athene noctua is decreasing in many European countries. In order to evaluate causes of the decline in Poland, habitat preferences of this species were analysed. Using GIS methods, 25 settled territories of the little owl, recorded during field surveys between 2000 and 2005, were compared with 50 unsettled locations. It was found that the proportion of built-up areas was higher in the occupied territories than in the random locations. No differences in grassland, forest and field proportion, habitat diversity and edge length were recorded between the occupied, and the random locations. The amount of forest and the proportion of built up areas appeared to be the best predictor of the occurrence of the little owl. Next, habitat use at 7 additional territories, which were occupied by little owls in 1980–90s and later abandoned, was analysed. In 2006, as compared to the period 1980–90, numbers of pollard willows decreased, whereas the number of buildings increased in these territories. The overall results lead to a conclusion that the little owl shows a high degree of habitat plasticity. The decrease of the area of grasslands and numbers of pollard willows is not likely to explain the population decline of the species.