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1. Effect of acute alcohol treatment on dopamine concentration in corpus striatum of rats: a voltammetric study

Creator:
Diaz, N. and Murgaš, K.
Type:
article, model:article, and TEXT
Subject:
rats, alcohol treatment, dopamine in striatum, and voltammetric determination
Language:
English
Description:
A voltammetry technique has been used to determine changes in dopamine release in the rat corpus striatum after two doses of ethanol administration. The dopamine oxidation current reached a maximal value at 30 min after the first alcohol dose with a subsequent decrease towards the initial level at 60 min and kept to the basal level with a statistically insignificant oscillation. When a second dose of alcohol was applied at 60 min, it was followed by a decrease of the dopamine oxidation current peak to 50 % of the initial value after another 60 min observation. The results resemble the known effect of alcohol on human behaviour (excitation followed by depression).
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

2. Effects of electroconvulsive shock on catecholamine release in the corpus striatum of the rat: a voltammetric study

Creator:
Pavlásek, J., Murgaš, K., Mašánová, C., and Haburčák, M.
Type:
article, model:article, and TEXT
Subject:
catecholamines, striatum, electroconvulsive stimulation, voltammetry, and rat
Language:
English
Description:
A voltammetric technique was used (differential pulse voltammetry with a carbon fibre microelectrode) to investigate dynamics of the changes of catecholamine overflow in the corpus striatum following electroconvulsive stimulation (ECS) of chloral hydrate-anaesthetized rats. Application of "maximal" ECS (50 Hz, AC, sine wave, approximately 150 mA, 0.2 s) caused large enhancement of catechol-oxidative current (CA.OC): In the first minute after its arrest, the CA.OC peak raised to 1032±405% (n=5, mean±S.D.) of the controls (P<0.001, Student's t-test). This large elevation of the extracellular catecholamine content ceased rapidly - the baseline level was attained in the second minute. CA.OC changes evoked by a "minimal" ECS (50 Hz, AC, sine wave, approximately 30 mA, 0.2 s) were equivocal in the first minute (increase, decrease: 145 ±56 %, P>0.05, n=6). Possible mechanisms of the ECS therapeutic effect are discussed.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Voltammetrically determined differences in changes evoked by KC1 microinjections on catecholamine levels in the reticular formation and corpus striatum of the rat

Creator:
Pavlásek, J., Mašánová, C., Bielik, P., and Murgaš, K.
Type:
article, model:article, and TEXT
Subject:
K+ depolarization, catecholamines, reticular formation, striatum, voltammetry, and rat
Language:
English
Description:
Using a microelectrode with carbon filaments and the voltammctric technique, changes evoked in the catechol oxidation current (CA.OC) and multiple unit activity (MUA) by microinjection of 3-5 ¿ri 03 mol.I'1 KC1 were studied in the reticular formation (RF) of the medulla oblongata of anaesthetized rats; the effect of KC1 stimulation of the RF and corpus striatum (S) on the CA.OC in these structures was compared. The microinjection of KC1 in the vicinity of the working electrode in the RF caused depression of MUA which began 2-3 s after administration, persisted for up to 6 min after and then diminished, reaching control values within 9 min. The voltammctric signal was first recorded in the 1st min after microinjection, when there was an evident decrease in the CA.OC value (59 % of the control value); this effect reached its maximum 7 min after administration (a mean drop to 23 % of the control), while at the end of the experiment (i.e. after 24 min) CA.OC values had risen to 45-80 % of the control value. The response in the S had a biphasic character, however. Immediately after the microinjection (1st min), the mean CA.OC value rose to 626 % of the control, while in the second phase (3-10 min) it was seen to fall below the control values (means 21-63 % of the control). The differences in the changes evoked by K+ depolarization in the concentration of catecholamines in the RF and S microenvironment are discussed from the aspect of the existence of different pools of the transmitter and other regional differences. The possibility of a relationship between considered.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public