Documented were the values of 7 morphological meristic and 37 mensural characters in samples of 0+ juvenile specimens of the sterlet reared in special aquacultural facilities in the Czech Republic (and originating from Russia), and in the Slovak Republic (originating from the Danube Slovak section). Individual character values were compared with literature data using the method size-pooled samples. Specimens reared in the Czech Republic (CR) and in the Slovak Republic (SR) did not differ in the maximum-minimum range of their meristic characters from the literature systematic description presented for sterlet (Acipenser ruthenus Linnaeus, 1758). Regards differences between mean values, the sample from the Czech Republic differed significantly in 4 (of 6) meristic characters, and in 14 (of 27) mensural characters compared. On the basis of multivariate morphometrical analysis (PCA, UPGMA) it was found that the sample of sterlet from the CR clusters well with other samples of the sterlet populations, being morphologically closest to a pair of samples from the Danube River. We suggest that, because of the numerous transfers into the Danube of sterlets reared by aquaculture in the CR, it will be impossible to discriminate them morphologically from those reared naturally in the Danube Slovak section.