Search

Start Over Creator Lotková, H.

2. Protective effect of S-adenosylmethionine against galactosamine-induced injury of rat hepatocytes in primary culture

Creator:
Kučera, O., Červinková, Z., Lotková, H., Křiváková, P. , Roušar, T., Mužáková , V. , Héžová, R., Kanďár , R. , and Rudolf , E.
Type:
article, model:article, and TEXT
Subject:
S-adenosylmethionine, Galactosamine, and Hepatocytes in vitro
Language:
English
Description:
The protective effect of S-adenosylmethionine (SAMe) on D-galactosamine (GalN)-induced damage to rat hepatocytes was tested in primary cultures. SAMe at concentrations of 50 and 1000 mg/l significantly reduced lactate dehydrogenase release from cells injured by 40 mM GalN after 24 h of incubation. There were no significant changes in urea production after 24 h among tested groups, including control hepatocytes. Exposure of hepatocytes to GalN leads to 3.5-fold decrease in urea synthesis after 48 h in comparison with control cell cultures. Addition of the highest dose of SAMe (1000 mg/l) into the culture media attenuated this decrease by 180 %. None of the tested doses of SAMe (5, 25, 50 and 1000 mg/l) affected considerably the reduced activity of mitochondrial dehydrogenases. The content of reduced and oxidized glutathione in GalN-exposed cells was diminished to 1.5 % and 16 %, respectively, of the control values after 24 h. Using only the highest concentration SAMe increased significantly these contents. SAMe had no effect on dramatically decreased albumin synthesis. These findings indicate beneficial effect of SAMe, especially of the highest concentration, on GalN-induced toxicity to rat hepatocytes in primary culture. This action of SAMe seems to be associated with reduction of plasma membrane damage and increased synthesis of glutathione.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Tert-butyl hydroperoxide selectively inhibits mitochondrial respiratory-chain enzymes in isolated rat hepatocytes

Creator:
Drahota, Z., Křiváková, P., Červinková, Z., Kmoníčková, E., Lotková, H., Kučera, O., and Houštěk, J.
Type:
article, model:article, and TEXT
Subject:
Hepatocytes, Mitochondrial enzymes, and tert-butyl hydroperoxide
Language:
English
Description:
Sensitivity of various mitochondrial enzymes to oxidative damage was tested on isolated rat liver hepatocytes permeabilized by digitonin. In permeabilized hepatocytes normal respiratory control values were obtained and mitochondrial membranes remained intact. Respiratory rate s of NADH-dependent (glutamate+malate, palmitylcarnitine + malate) and flavoprotein-dependent (succinate) substrates were determined in hepatocytes exposed for 5 min to 0.5-3 mM tert -butyl hydroperoxide before addition of digitonin. Our data showed that oxidation of NADH-dependent substrates is much more sensitive to oxidative stress than ox idation of flavoprotein-dependent ones, evidently due to the modification of iron-sulfur clusters or SH groups in the NADH dehydrogenase enzyme complex (Complex I).
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public