In northwestern Spain, the spatial distribution of Iberian wolf scats left during the reproductive period in the den area and other zones of the territory was analysed. In the den area, a large number of scats were left on inconspicuous substrates and at ground level, whereas scats were left mainly on conspicuous substrates and above-ground level in the rest of the territory. In the den area the number of scats detected in the centre, tire, and lateral sections of the roads was identical. In the rest of the territory, scats were left in the lateral section of the roads. The distribution of the scats on the access trail to the den area site showed characteristics that were intermediate between the other two areas. The results of site analysis suggest that the scats left in the den surroundings and areas removed from the den area have a function in the wolf’s scent-marking and visual signalling. They are not left at random, but at points and on substrates that enhance their effectiveness as olfactory and visual marks. The abundance of scats left in the den area were only a consequence of continuous passage through the area.