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Start Over Creator Černá, Kateřina

2. Identification of 37 microsatellite loci for Anthophora plumipes (Hymenoptera: Apidae) using next generation sequencing and their utility in related species

Creator:
Černá, Kateřina and Jakub Straka
Type:
article, články, model:article, and TEXT
Subject:
Zoologie, zoologie, entomologie, Hymenoptera, Apidae, microsatellite development, Anthophora plumipes, 454 sequencing, Anthophorini, 2, and 59
Language:
English
Description:
Novel microsatellite markers for the solitary bee, Anthophora plumipes, were identified and characterised using 454 GS-FLX Titanium pyrosequencing technology. Thirty seven loci were tested using fluorescently labelled primers on a sample of 20 females from Prague. The number of alleles ranged from 1 to 10 (with a mean of 4 alleles per locus), resulting in an observed heterozygosity ranging from 0.05 to 0.9 and an expected heterozygosity from 0.097 to 0.887. None of the loci showed a significant deviation from the Hardy-Weinberg equilibrium and only two loci showed the significant presence of null alleles. No linkage between loci was detected. We further provide information on a single multiplex PCR consisting of 11 of the most polymorphic loci. This multiplex approach provides an effective analytical tool for analysing genetic structure and carrying out parental analyses on Anthophora populations. Most of the 37 loci tested also showed robust amplification in five other Anthophora species (A. aestivalis, A. crinipes, A. plagiata, A. pubescens and A. quadrimaculata). The result of this study demonstrates that next generation sequencing technology is a valuable method for isolating quality microsatellites in non-model species of solitary bees. and Obsahuje seznam literatury
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

3. Two ways of experimental infection of Ixodes ricinus ticks (Acari: Ixodidae) with spirochetes of Borrelia burgdorferi sensu lato complex

Creator:
Fišerová, Lenka, Černá, Kateřina, Horká, Helena, and Kopecký, Jan
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Lyme disease spirochete, Borrelia burgdorferi, Borrelia garinii, Borrelia afzelii, tick, and Ixodes ricinus
Language:
English
Description:
A previously reported procedure for the introduction of Borrelia spirochetes into tick larvae by immersion in a suspension of spirochetes was tested on Ixodes ricinus (L.) ticks and three of the most medically important European Borrelia genomic species, B. burgdorferi sensu stricto, B. garinii and B. afzelii. The procedure was compared with ''classical'' infection of nymphs by feeding on infected mice. Both methods yielded comparable results (infection rate 44-65%) with the exception of B. afzelii, which produced better results using the immersion method (44%) compared with feeding on infected mice (16%). Nymphs infected by the immersion method at the larval stage were able to transmit the infection to naïve mice as shown by serology and PCR detection of spirochetal DNA in organs. The immersion method is faster than feeding on infected mice and provides more reproducible conditions for infection. It can be exploited for studies on both pathogen transmission and Borrelia-vector interactions.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public