In Hungary, during the 2000s, pesticide poisoning became the most important threat for raptors, especially for the globally threatened Eastern imperial eagle (Aquila heliaca). In September 2013, with a focus on carbofuran and phorate, the first poison and carcass detection dog (PCDD) unit was formed in Hungary with a specifically trained detection dog and handler. Two more dogs were subsequently trained and joined the unit in 2017 and 2020 respectively. Between its inception until August 2020, the PCDD unit conducted 1,083 searches in five countries, which revealed 329 poisoned animals of 15 bird and nine mammal species, 120 poisoned baits and five pesticide products. Globally threatened species, including eight Eastern imperial eagles and four saker falcons (Falco cherrug), were among the detected victims. Present at 66.45% of wildlife poisoning events, the unit revealed 37.87% of the victims and 79.70% of the poisoned baits known in Hungary during the period 2013-2020. Compared to human surveys, the PCDD unit demonstrated a significantly higher find rate for poisoned baits. At 22 poisoning events (14.38% of all cases) only the PCDD unit revealed victims or poisoned baits; cases that would probably have gone undetected without the PCCD unit. Of the two focal pesticides, carbofuran was more frequently detected – in 88.56% of the positive samples. The unit played a significant role in detecting and combating wildlife poisoning incidents by deterring potential offenders and facilitating police investigations through retrieval of evidence otherwise difficult to obtain.