A new coccidian parasite of the genus Caryospora is described from a long nosed vine snake, Ahaetulla nasuta, Lacépède, 1789 from Thailand. Oocysts of Caryospora ahaetullae sp. n. are spherical or slightly subspherical, 24.8 (22.5-26.0) pm in diameter, with a single-layered wall about 0.75 pm thick. A micropyle or oocyst residuum is absent. One, rarely two, polar granules are present, 1.5-2.0 pm. Sporocysts are ellipsoidal, 18.5 x 14.2 (18.0-19.5 x 13.0-15.0) pm with prominent Stieda and substieda bodies. Sporocyst residuum present, consisting of numerous very small granules scattered among 8 sporozoites. Sporo-zoites, with prominent transverse striations anteriorly, are vermiform, 15.0 x 3.8 (13.5-16.5 x 3.5—4.5) pm (in situ), lying lengthwise and parallel within sporocyst. Each sporozoite contains a spherical anterior and spherical to subspherical posterior refractile bodies.
Anticoccidial efficacy of a drug combination containing monensin at 8 p.p.m. plus the new antioxidant duokvin at 120 p.p.m. in the feed was compared with that of monensin alone at the recommended level of 100 p.p.m. against a field isolate of the coccidium Eimeria acervulina Tyzzer, 1929 in a battery study. Both monensin and monensin duokvin combination were effective against E. acervulina when judged by weight gain, feed conversion and faecal scores. There was no significant difference in the chemoprophylactic activity of either treatments. Neither monensin at 100 p.p.m. nor the combination proved effective in terms of oocyst production. In accordance with the earlier findings with E. lenella, the combination seems appropriate for field trials.
The paper presents the current possibilities of the environmental electron microscopy laboratory at the ISI CAS in Brno in imaging epoxy resin surface nanostructures with nanoparticles. The results show morphological artifacts emerging due to sample coating or its evaporation caused by the thermal effects of the electron beam, and further provide a comparison between samples observed under the conditions approaching vacuum and samples observed under a relatively high gas pressure in the environmental scanning electron microscope. Samples are displayed in a completely natural state in a relatively high gas pressure and, given the environmental conditions, at a very high resolution in a modified ESEM QUANTA 650 FEG. and Článek prezentuje současné možnosti laboratoře environmentální elektronové mikroskopie ÚPT AVČR v Brně při zobrazování povrchových nanostruktur epoxidové pryskyřice s nanočásticemi. Výsledky poukazují na morfologické artefakty vznikající pokovením vzorku či jeho odpařováním vlivem tepelných účinků elektronového svazku a dále přinášejí možnost porovnání zobrazení vzorků v podmínkách blížících se vakuu a při relativně vysokém tlaku plynů environmentálního rastrovacího elektronového mikroskopu. Vzorky jsou v podmínkách relativně vysokého tlaku plynů zobrazovány ve zcela přirozeném stavu a vzhledem k tomu s velmi vysokým rozlišením pomocí upraveného EREM QUANTA 650 FEG.