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Start Over Coverage 7-16

1. A digenean parasite in a mudskipper: Opegaster ouemoensis sp. n. (Digenea: Opecoelidae) in Periophthalmus argentilineatus Valenciennes (Perciformes: Gobiidae) in the mangroves of New Caledonia

Creator:
Bray, Rodney A. and Justine, Jean-Lou
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Opegaster review, helminths, Nematoda, and Acanthocephala
Language:
English
Description:
Opegaster ouemoensis sp. n. is described from Periophthalmus argentilineatus Valenciennes (Gobiidae). Distinctive features included the weak or undetectable papillae of the ventral sucker and the small, but distinct cirrus-sac. The new species is compared with 25 marine species of Opegaster for which a table of measurements and ratios is presented. The new combination Opegaster queenslandicus (Aken'Ova, 2007) (originally in Opecoelus) is formed. Fifteen mudskippers were intensively examined for parasites; larval anisakid nematodes and acanthocephalans were found, but no monogeneans, cestodes, copepods, isopods, hirudineans or adult nematodes. A brief summary of the helminth parasites of mudskippers is included.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Comprehensive analysis of PTEN in primary cutaneous melanoma

Creator:
Němejcová, K., Dundr, P., Jakša, R. , Bártů, M. , Stružinská, I., Hojný, J. , Hájková, N., and Kodet, O.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
melanoma, mutation, deletion, loss of expression, and PTEN
Language:
English
Description:
Phosphatase and tensin homologue (PTEN) is a tumour suppressor gene implicated in tumorigenesis of melanoma, with distinct cytoplasmic and nuclear functions. Cytoplasmic PTEN negatively regulates the PI3K/AKT/mTOR signalling pathway, while nuclear PTEN works as a tumour suppressor. Clinical data suggest that the loss of PTEN function in melanoma is associated with aggressive tumour behaviour. We performed a comprehensive analysis of PTEN in 112 primary cutaneous melanomas including immunohistochemical (IHC), fluorescent in situ hybridization (FISH), next-generation sequencing (NGS), and epigenetic analysis. The goal of our study was to: (a) correlate PTEN expression with selected clinico-pathological variables, and assess its prognostic significance; (b) correlate molecular aberrations with PTEN expression to consider the utility of immunohistochemical analysis of PTEN protein expression for screening PTEN genetic alterations; (c) review the literature and evaluate the PTEN expression level in melanoma with respect to possible therapeutic targeting. Our results showed that PTEN molecular alterations were present in 4/20 (20 %) cases with a loss of expression, 3/11 (27 %) cases with clonal-like expression, and 1/81 (1 %) cases with positive PTEN expression. No PTEN promoter methylation was found in any of the cases. Even though the value of our observation is limited by the low number of cases fully evaluated by IHC (112 cases), FISH (19 cases) and NGS (30 cases), our data suggest that IHC is not an appropriate method for the screening of PTEN genetic alterations. Our survival analysis suggests that patients with positive cytoplasmic PTEN expression show better disease-free survival (P < 0.05).
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

4. Spectroscopic investigation of chlorophyll pigments in anhydrous polymer matrices

Creator:
Wróbel, D., Van Zandvoort, M.A.M.J., Van Ginkel, G., and Levine, Y.K.
Format:
print
Type:
model:internalpart and TEXT
Language:
Multiple languages
Description:
The properties of chlorophyll (Chl) a and pheophytin (Pheo) a embedded in the rigid anhydrous polymer fihns, polyvinyl alcohol (PVA) and nitrocellulose (NC) are reviewed. The aggregation capability of these pigments in two matrices is characterized by absorption, fluorescence, excitation spectra combined with fluorescence lifetime and time-resolved anisotropy. The dimer creation ability for Chl a and Pheo a is strongly affected by the polymer systém ušed for investigation. The Chl a and Pheo a ability to aggregate in polymer matrices is compared. We háve been looking for a good systém suitable for the study of properties of monomeric forms when embedded in the rigid matrix on the one hand, and for the study of energy transfer between different forms of Chl molecules on the other one. Chl a, when embedded in an anhydrous PVA matrix, is in a very stable monomeric form while Pheo a molecules in this film tend to aggregate and in the NC film they exist in the monomeric form. Thus the PVA systém can be ušed as a model systém for investigation of the properties of and processes between both dimeric and monomeric structures. The NC film, however, can be ušed as a rigid matrix when only the existence of monomeric forms of the pigments is required.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public