French bean (Phaseolus vulgaris L.) cotyledons lost most of their reserve substances during several early days of germination and turned green. In cotyledon mesophyll cells of one-week-old seedlings, plastids were represented predominantly by amyloplasts (starch grains) and chloroamyloplasts, and the cells appeared to be metabolically highly active. Cell heterogeneity associated with distance of the cells from cotyledon vascular bundles was evident. Only mesophyll cells near to the bundles were rich in plastids. In two-weeks-old intact bean plants, the cotyledons were yellow and shrunken, and their cells were nearly "empty". The plastids in them were represented by senescent plastids (gerontoplasts) only. In the gerontoplasts as well as freely in cytosol, fluorescent lipoid inclusions were accumulated. This cotyledon development was more or less independent of irradiance. In "decapitated" bean plants, senescence of mesophyll cells and plastids was slowed down considerably, and the life span of the cotyledons was prolonged. and J. Kutík, N. Wilhelmová, J. Snopek.
In greenhouse experiments the efFect of simulated acid rain (mist of H2SO4) treatment on i'*C02 fixation, ribulose-l,5-bisphosphate carboxylase (RuBPC), nitráte reductase (NR) and nitrite reductase (NiR) activities in Vigna sinensis L. and Phaseolus mungo L. was investigated at different pH. Seedlings exposed to acidic mists of pH 5.6, 4.0 and 2.0 for 5 d showed significant reduction in *“*€02 fixation, RuBPC, NiR and especially NR activities. Sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE) of crude leaf extracts of RuBPC indicated a significant loss of 55 and 15 kDa polypeptides at pH 2.0 in both the crop plants. The reduction in the RuBPC activity in seedlings grown under acidic mists correlated well with the CO2 fixation. NR activity was affected more than the NiR activity.
Both the synthesis of apoprotein P700 with molecular mass of 72 kDa and asseinbly of chlorophyll (Chl) a-protein of photosystem (PS) 1 were blocked by lincoinycin (inhibitor of translation in chloroplasts). Synthesis of 48 and 43,5 kDa polypeptides belonging to the PS2 complex were also suppressed. Chl a forms of PS2 reaction centi e (RC) at 683 nin and of PS 1 RC at 690, 696 and 705 nm disappeared, Relative Chl h content and fluorescence bands ratio F736/F686 increased significantly Cycloheximide (inhibitor of translation in cytoplasin) ti eatinent resulted in reduction of polypeptides of the light-harvesting Chl a//i-protein complex (LFIC) in the 28.5- 25.5 kDa region. At the same time a relatively smaller decrease was observed of two Chl foirns of the LHC: Chl by 3.8 % and Chl by 10.2 % (12.4 and 34.3 % in the contiol, respectively). The fluorescence bands ratio F736/F686 decrease in low temperature spectra was possibly connected with the LIlC-1 and LIlC-2 synthesis blocking.
We study a particular digraph dynamical system, the so called digraph diclique operator. Dicliques have frequently appeared in the literature the last years in connection with the construction and analysis of different types of networks, for instance biochemical, neural, ecological, sociological and computer networks among others. Let D=(V,A) be a reflexive digraph (or network). Consider X and Y (not necessarily disjoint) nonempty subsets of vertices (or nodes) of D. A disimplex K(X,Y) of D is the subdigraph of D with vertex set X ∪ Y and arc set {(x,y)∶ x ∈ X, y ∈ Y\} (when X ∩ Y ≠ ∅, loops are not considered). A disimplex K(X,Y) of D is called a diclique of $D$ if $K(X,Y)$ is not a proper subdigraph of any other disimplex of D. The diclique digraph $\overrightarrow {k}(D) of a digraph $D$ is the digraph whose vertex set is the set of all dicliques of $D$ and ( K(X,Y),K(X',Y'))$ is an arc of $\overrightarrow {k}(D) if and only if Y ∩ X' ≠ ∅. We say that a digraph $D$ is self-diclique if $\overrightarrow {k}(D)$ is isomorphic to D. In this paper, we provide a characterization of the self-diclique circulant digraphs and an infinite family of non-circulant self-diclique digraphs.