Dynamic changes of neoxanthin (NEO), violaxanthin (VIO), anteraxanthin (ANT), zeaxanthin (ZEA), chlorophyll (Chl) a, Chl b, α-carotene, β-carotene, and their behaviour under increasing duration of high irradiance (HI) were investigated in the soybean hypocotyl callus culture. The calli were induced on solid (1.1 % agar) MS medium (pH 5.8) supplemented with 4.52 μM 2,4-D, 2.32 μM kinetin, and 3 % sucrose. After 30 d of culture, the green calli were irradiated with "white light" (133W m-2) for 0, 3.5, and 24 h. HPLC profiles were separated on a C18 column. With increasing duration of HI, the content of total carotenoids (Cars) increased, but the ratio of Chl a+b/Cars decreased. With lengthening the duration of HI, there was induction of ZEA. Contents of ANT, α-carotene, and β-carotene remained nearly constant, but ratio of ZEA/Chl a+b increased with lengthening the HI duration. and D. M. Pandey, K. H. Kim, U. D. Yeo.
Motto: "Pevnolátkové osvětlování je dnes tam, kde byl Internet v 80. letech. Stejně jako jsme tehdy nemohli předpovědět, jaký bude Internet, dnes, o 30 let později, nedokážeme předvídat, co všechno se stane se světelnou technikou a osvětlováním v příštích dekádách. Víme jen, že to bude úžasné a krásné." Roland Haitz, During its long evolution, techniques for artificial lighting used four main principles: combustion, incandescence, gas discharge and luminescence. The injection electroluminescence (taking place in light-emitting diodes, LED) is probably the most efficient way of converting electricity into light. In this paper we describe the main advantages and disadvantages of LED based light sources, as well as some topics of current research such as laser based light sources. Finally, the possible health effects of artificial lighting are briefly discussed., Jan Valenta, Ivan Pelant., and Obsahuje seznam literatury
The glutathione S-transferases (GSTs) are a family of multifunctional enzymes involved in cellular detoxification. The aim of this study was to evaluate the effect of albendazole - drug of choice for trichinellosis - on the total activity and kinetics of cytosolic GST in the mouse intestines during experimental trichinellosis. Our results showed a statistically significant decrease in the total GST activity both in the small and large intestines of the mice infected with the nematode Trichinella spiralis (Owen, 1835) and treated with albendazole, compared with the control mice that were infected but untreated with the drug. Furthermore, albendazole administration modified the kinetics of substrate saturation of GST in the intestines of the infected mice because the drug caused changes in Michaelis constant values of this enzyme. Based on our observations, we suggest that the quaternary structure of GST from the mouse intestines is impacted by this drug during trichinellosis.