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2. Formation of cross-linking between photosystem 2 proteins during irradiation of thylakoid membranes at high temperature

Creator:
Singh, Abhay K. and Singhal, G. S.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
D1 protein, diphenylcarbazide, oxygen evolving complex, scavengers, Triticum aestivum, and wheat
Language:
Multiple languages
Description:
Irradiation of thylakoid membranes at 40 °C resulted in complete inhibition of photosystem (PS) 2 activity measured as 2,6-dichlorophenol indophenol (DCIP) photoreduction either in the absence or presence of 1,5-diphenylcarbazide (DPC). Concomitant with the inactivation of PS2 activity, several thylakoid proteins were lost and high molecular mass cross-linking products appeared that cross-reacted with antibodies against proteins of PS2 but not with antibodies against proteins of other three complexes PS1, ATP synthase, and cytochrome b6f. Irradiation of thylakoid membranes suspended in buffer of basic pH or high concentration of Tris at 25 °C resulted in the formation of cross-linking products similar to those in thylakoid membranes irradiated at 40 °C. Presence of radical scavengers and DPC during the high temperature treatment prevented the formation of cross-linking products. These results suggest the involvement of oxygen evolving co mplex (OEC) in the formation of cross-linking between PS2 proteins in thylakoid membrane irradiated at high temperature. and Abhay K. Singh, G. S. Singhal.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public