Acclimatization of micropropagated plantlets induces an antioxidative burst: a case study with Ulmus minor Mill.

Title:

Acclimatization of micropropagated plantlets induces an antioxidative burst: a case study with Ulmus minor Mill.

Creator:

Dias, M. C., Pinto, G., and Santos, C.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fde05930-a285-4384-99f7-c31528d5bea1
uuid:fde05930-a285-4384-99f7-c31528d5bea1
issn:0300-3604
doi:10.1007/s11099-011-0028-9

Subject:

botanika, botany, antioxidant enzymes, lipid peroxidation, micropropagation, and Ulmus minor

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

In this article, the effects of increased light intensities on antioxidant metabolism during ex vitro establishment of Ulmus minor micropropagated plants are investigated. Three month old in vitro plants were acclimatized to ex vitro conditions in a climate chamber with two different light intensities, 200 μmol m-2 s-1 (high light, HL) and 100 μmol m-2 s-1 (low light, LL) during 40 days. Immediately after ex vitro transfer, the increase of both malondialdehyde (MDA) and electrolyte leakage in persistent leaves is indicative of oxidative stress. As the acclimatization continues, an upregulation of the superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR) enzyme activities were also observed. Simultaneously, MDA content and membrane permeability stabilized, suggesting that the antioxidant enzymes decrease the deleterious effects of reactive oxygen species (ROS) generation. Unexpectedly, newly formed leaves presented a different pattern of antioxidative profile, with high levels of MDA and membrane leakage and low antioxidant enzyme activity. Despite these differences, both leaf types looked healthy (e.g. greenish, with no necrotic spots) during the whole acclimatization period. The results indicate that micropropagated U. minor plantlets develop an antioxidant enzyme system after ex vitro transfer and that, in general, LL treatment leads to lower oxidative stress. Moreover, new leaves tolerate higher levels of ROS without the need to activate the antioxidative pathway, which suggests that the environment at which leaves are exposed during its formation determinate their ability to tolerate ROS. and M. C. Dias, G. Pinto, C. Santos.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

259-266

Source:

Photosynthetica | 2011 Volume:49 | Number:2

Harvested from:

CDK

Metadata only:

false