Chloroplast protease/chaperone AtDeg2 holds γ1 subunit of ATP synthase in an unaggregated state under high irradiance conditions in Arabidopsis thaliana

Title:

Chloroplast protease/chaperone AtDeg2 holds γ1 subunit of ATP synthase in an unaggregated state under high irradiance conditions in Arabidopsis thaliana

Creator:

Jagodzik, P. and Jackowski, G.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fdd78b5e-5844-42e4-8f80-04b8f2f09e87
uuid:fdd78b5e-5844-42e4-8f80-04b8f2f09e87
doi:10.32615/ps.2022.004

Subject:

chaperone, Deg2, elevated irradiance, homodimerization, and protease

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Little data on the role played in vivo by chloroplast protein AtDeg2 as a chaperone is available. Therefore, we sought for chloroplast proteins protected from high irradiance-induced interprotein aggregation via disulphide bridges by AtDeg2 acting as a holdase. To reach this goal, we performed analyses which involved comparative diagonal electrophoreses of lysates of chloroplasts isolated from wild type (WT) plants and transgenic plants 35S:AtDEG2ΔPDZ1-GFP which expressed AtDeg2 lacking its chaperone activity but retaining the protease activity. The results of the analyses indicate that AtDeg2 acting as a holdase prevents a single chloroplast protein, i.e., the γ1 subunit of ATP synthase from long-term high irradiance-induced homodimerization mediated by disuplhide bridges and this allows us to better understand a complexity of physiological significance of AtDeg2 - the chloroplast protein of dual protease/chaperone activity.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

212-218

Source:

Photosynthetica | 2022 Volume:60 | Number:2

Harvested from:

CDK

Metadata only:

false