FSH, oxytocin and IGF-I regulate the expression of sirtuin 1 in porcine ovarian granulosa cells

Title:

FSH, oxytocin and IGF-I regulate the expression of sirtuin 1 in porcine ovarian granulosa cells

Creator:

Sirotkin, Alexander V., Dekanová, Petra, and Harrath, Abdel Halim

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fb3f2b96-25c9-4f0a-ad6b-02c96af88af6
uuid:fb3f2b96-25c9-4f0a-ad6b-02c96af88af6
doi:10.33549/physiolres.934424

Subject:

pig, ovarian granulosa cells, Sirtuin 1, FSH, oxytocin, and IGF-I

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

The involvement of the mTOR system/enzyme sirtuin 1 (SIRT1) intracellular signaling system in the control of ovarian functions and its role in mediating hormonal action on the ovary has been proposed, but this hypothesis should be supported by a demonstrated influence of hormones on mTOR/SIRT1. Therefore, the aim of our in vitro experiments was to examine the effect of the known hormonal regulators of ovarian functions, such as follicle-stimulating hormone (FSH), oxytocin (OT) and insulin-like growth factor I (IGF-I), on mTOR/SIRT1. The accumulation of SIRT1 in porcine ovarian granulosa cells cultured with and without these hormones (at doses of 1, 10 or 100 ng.ml-1 ) was evaluated using immunocytochemistry. It was observed that the addition of FSH (at 10 ng.ml-1 but not at 1 or 100 ng/ml) and OT (at all tested doses) increased the expression of SIRT1 in ovarian cells. In addition, 100 ng.ml-1 , but not at 1 or 10 ng.ml-1 , of IGF-I decreased SIRT1 accumulation. Our observations are the first demonstration that hormones can directly regulate the ovarian mTOR/SIRT1 system and that this system could mediate the action of hormonal regulators on the ovary.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

461-466

Source:

Physiological research | 2020 Volume:69 | Number:3

Harvested from:

CDK

Metadata only:

false