Identification of photosynthetic mutants of Arabidopsis by automatic screening for altered effective quantum yield of photosystem 2

Title:

Identification of photosynthetic mutants of Arabidopsis by automatic screening for altered effective quantum yield of photosystem 2

Creator:

Varotto, C., Pesaresi, P., Maiwald, D., Kurth, J., Salamini, F., and Leister, D.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ed8e241c-4abc-4e66-a884-cf1877900173
uuid:ed8e241c-4abc-4e66-a884-cf1877900173
issn:0300-3604
doi:10.1023/A:1012445020761

Subject:

chlorophyll fluorescence, electron transport, and gene tagging

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Quantification of chlorophyll (Chl) fluorescence is a versatile tool for analysing the photosynthetic performance of plants in a non-intrusive manner. A pulse-amplitude modulated fluorometer was combined with a CNC router for the automated measurement of the effective quantum yield of photosystem 2 (Φ2) of Arabidopsis thaliana plants. About 90 000 individual plants representing 7 500 lines derived from En-transposon and T-DNA mutagenised Arabidopsis populations were screened for mutants with altered Φ2. Forty-eight recessive Φ2 mutations were identified of which most exhibit also altered pigmentation and increased photosensitivity. For three Φ2 mutants the corresponding mutated genes were identified that code all for chloroplast-located proteins. Comparison of the Φ2 mutant screen with other screening methods based on the measurement of Chl fluorescence shows that the Φ2 mutants identified are different to mutants identified by high Chl fluorescence. Some Φ2 mutants, on the contrary, are common to mutants identified by screens based on non-photochemical quenching. and C. Varotto ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

497-504

Source:

Photosynthetica | 2000 Volume:38 | Number:4

Harvested from:

CDK

Metadata only:

false