Native high-density lipoprotein and melatonin improve platelet repsonse induced by glycated lipoproteins

Title:

Native high-density lipoprotein and melatonin improve platelet repsonse induced by glycated lipoproteins

Creator:

Ozsavci, D., Nazli, A., Bingol Ozakpinar, O., Yanikkaya Demirel, G., Vanizor Kural, B., and Sener, A.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ec7d009d-f6e2-45d0-b9af-fb7c06d9443e
uuid:ec7d009d-f6e2-45d0-b9af-fb7c06d9443e

Subject:

high-density lipoproteins, in vitro glycation, low-density lipoproteins, melatonin, and platelets

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Activated platelets and glycated lipoproteins are responsible for atherothrombosis in diabetics. Melatonin and native high-density lipoproteins are crucial in the preservation of pro/oxidant- antioxidant balance. The aim of the present study was to investigate the in vitro effects of native high-density lipoproteins and melatonin on altering th platelet response induced by glycated lipoproteins. Low-density lipoproteins and high-density lipoproteins were purified from plasma by ultracentrifugation and were glycated with glucose for three weeks. After incubation with or without melatonin/or native high-density lipoproteins, low-density lipoproteins, glycated low-density lipoproteins/glycated high-density lipoproteins were added to ADP-induced platelets. Oxidative parameters, caspase-3/9 and nitric oxide levels were measured spectrophotometrically; CD62-P/ annexin-V expression was determined by flow cyto-metry. In glycated low-density lipoprotein/glycated high-density lipoprotein-treated groups, platelet malondialdehyde/protein carbonyl, P-selectin, annexin-V, caspase-3/9 levels were increased (ranging from P < 0.001 to P < 0.01); glutathione and nitric oxide levels were reduced (ranging from P < 0.001 to P < 0.01). In glycated low-density lipoprotein/glycated high-density lipoprotein-treated groups, melatonin treatment reduced malondialdehyde, protein carbonyl, CD62-P, annexin-V and caspase-3/9 (P < 0.001, P < 0.01) levels and elevated nitric oxide (only glycated low-density lipoproteins). In glycated low-density lipoprotein/gly-cated high-density lipoprotein-treated groups, native high-density lipoprotein treatment reduced malondi-aldehyde, protein carbonyl, annexin-V, caspase-3/9 levels (P < 0.001, P < 0.01) and increased glutathione; nitric oxide levels (only with gly-HDL). Both melatonin and high-density lipoproteins should be regarded as novel promising mechanism-based potential therapeutic targets to prevent atherothrombosis in diabetics. and Corresponding author: Derya Ozsavci

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

[144]-152

Source:

Folia biologica | 2018 Volume:64 | Number:4

Harvested from:

CDK

Metadata only:

false